Guven Lab. on Therapeutic Bioengineering

OVERVIEW

Advances in stem cell biology and genetics with bioengineering science continuously lead breakthroughs and innovations in biotechnology. We harmonize fundamental sciences such as chemistry, biology, and physics with principles of engineering to generate innovative and effective therapeutic approaches, tissue mimicries and medical microdevices for medicine and pharmaceutical sciences. Our group focuses on bioengineering of novel native-like 3D cellular microenvironments, organ-on-a-chip platforms, and cell therapy approaches and integrates them for human health.

RESEARCH INTERESTS

Tissue and cellular microenvironment engineering: An understanding of how stem cells respond to physical and chemical stimulations from their extracellular environment is essential for utilizing the therapeutic potential of stem cells. We focus on developing novel biomaterials to construct cellular niches that can provide control over the stem cell differentiation and functionalization. We build tissues for regenerative medicine and pharmaceutical applications by designing and defining the cell-material and cell-cell interactions. Utilizing the bioengineering principles we develop bioreactor systems, tissue fabrication and assembly techniques for bottom-up and top-down tissue engineering.

Bio-mimicry organ-on-a-chip platform technologies for biomedical research: Organs-on-chips technologies provide a great platform for the investigation of fundamental mechanisms of organ physiology and disease. Such tools enable control over the physical and chemical factors of an in vitro organ models. Our group focuses on designing and engineering BioMEMS for basic research on cell physiology, in vitro disease models (e.g., neurodegenerative diseases, rare diseases, and cancer), diagnostics and pharmaceutical research.

Personalized and cellular therapies: Cell-based therapies provide unique and personalized approaches in current clinical treatments. Autologous and allogenic adult mesenchymal stem cells from bone marrow, adipose tissue, Wharton jelly and dental pulp, as well as limbal stem cells, and specialized cells (e.g., chondrocytes, hepatocytes) have been demonstrated to be effective in clinics. We develop novel therapeutic methods from bench to bedside by utilizing of cell-based therapies and intraoperative approaches in clinics.

RESEARCH HIGHLIGHTS

Biological organizations are highly hierarchical in architecture from the molecular to the macroscopic level. Such complexity in tissues is formed by building units that regulate and provide the system function. To mimic and bioengineer functional tissues, we need biomanufacturing tools that can generate and manipulate multi-scale building blocks. Bottom-up tissue engineering approaches focus on creating tissues by assembling heterogeneous building units such as biomacromolecules, cells, and cell-loaded microscale hydrogels in a multiscale manner. In our recent works, we demonstrated different cell and tissue assembly approaches utilizing acoustic, magnetic, micro robotic and bioprinting technologies to generate model platforms and tissues. Bioengineering approaches are essential for current therapeutic and diagnostic applications in medicine. Together with advances in genetics and cell biology, bioengineering tools provide key elements for novel and innovative medical technologies for human health.

Engineering platforms and systems for biofabrication of 3D tissues and microenvironments.

Representative bioengineered tissue constructs A. Engineering of large osteogenic grafts with rapid engraftment capacity using mesenchymal and endothelial progenitors from human adipose tissue (Guven et al. 2011), B. In vitro endothelial capillaries from stromal vascular fraction (Guven et al. 2011), C. Enhanced engraftment and functionality of bio-engineeredautologous dermo-epidermal skin grafts pre-vascularized with adipose-derived cells (Klar et al. 2014), D. Direct neural differentiation of mouse embryonic stem cells in 3D microenvironments, E. Biotunable acoustic node assembly of hepatic organoids (Chen et al. 2015), F. Guided and magnetic self-assembly of tunable magnetoceptive cardiac microtissues (Tasoglu et al. 2014).

Group Members

Guven Lab. on Therapeutic Bioengineering

Group Leader

Sinan GUVEN
sinan.guven@ibg.edu.tr
+90 232 299 41 00 (5131)
+9 02322994163

Asli YILDIRIM Senior Researcher asli.yildirim@ibg.edu.tr

Münire Ekmekçigil Research Associate munire.ekmekcigil@ibg.edu.tr

Ali Kemal Baş MSc Student

Olcay Burçin Akbulud MSc Student

Melis Asal MSc Student

Emine Kahraman MSc Student

İbrahim Halilullah Erbay MSc Student ibrahimhalilullah.erbay@msfr.ibg.edu.tr

Selected Publications

Utine CA, Ayhan Z, Durmaz Engin C. Effect of intracorneal ring segment implantation on corneal asphericity. Int J Ophthalmol. 2018 Aug ; 11 (8): 1303-1307. doi:10.18240/ijo.2018.08.09.

Utine CA, Durmaz Engin C, Ayhan Z. Effects of Preoperative Topometric Indices on Visual Gain After Intracorneal Ring Segment Implantation For Keratoconus. Eye & Contact Lens Science and Clinical Practice. 2018 Nov ; 44 (2): S387-S391. doi:10.1097/ICL.0000000000000490.

Raciha Beril Küçümen, Berna Şahan, Canan Aslı Yıldırım, Ferda Çiftçi. Evaluation of Corneal Biomechanical Changes After Collagen Crosslinking in Patients with Progressive Keratoconus by Ocular Response Analyzer. Turk J Ophthalmol. 2018 Aug ; 48 (4): 160-165. doi:10.4274/tjo.56750.

Utine CA, Stern M, Akpek EK. Immunopathological Features of Severe Chronic Atopic Keratoconjunctivitis and Effects of Topical Cyclosporine Treatment. Ocular immunology and inflammation. 2018 Sep ; 6 1-10. doi:10.1080/09273948.2018.1511811.

Çakir H, Utine CA. Management of Reverse Pellucid Type Topography. Eye & contact lens. 2018 Nov ; 44 (Suppl 2): S131-S136. doi:10.1097/ICL.0000000000000423.

Kocaoğlu G, Utine CA, Yaman A, Men S. Orbital Apex Syndrome Secondary to Herpes Zoster Ophthalmicus.. Turkish journal of ophthalmology. 2018 Feb ; 48 (1): 42-46. doi:10.4274/tjo.02256.

Arikan G, Karatas E, Lebe B, Ayhan Z, Utine CA, Kutsoylu OE, Gunenc U, Yilmaz O. Topically applied 1% voriconazole induces dysplastic changes on the ocular surface: animal study. Cutaneous and ocular toxicology. 2018 Dec ; 37 (4): 328-331. doi:10.1080/15569527.2018.1463237.

Utine CA, Engin Durmaz C, Koçak N. Corneal matrix repair therapy with the regenerating agent in neurotrophic persistent epithelial defects. International journal of ophthalmology. 2017 Dec ; 10 (12): 1935-1939. doi:10.18240/ijo.2017.12.25.

Mathews PM, Ramulu PY, Swenor BS, Utine CA, Rubin GS, Akpek EK. Functional impairment of reading in patients with dry eye. The British journal of ophthalmology. 2017 Apr ; 101 (4): 481-486. doi:10.1136/bjophthalmol-2015-308237.

Utine CA. Keratoprotez. MN Oftalmoloji. 2017 ; 24 (1): 115-124.

Zhu Y, Serpooshan V, Wu S, Demirci U, Chen P, Güven S. Tissue Engineering of 3D Organotypic Microtissues by Acoustic Assembly. Methods Mol Biol. . 2017 Sep doi:10.1073/pnas.1708621114.

Calibasi Kocal G, Güven S, Foygel K, Goldman A, Chen P, Sengupta S, Paulmurugan R, Baskin Y, Demirci U. Dynamic Microenvironment Induces Phenotypic Plasticity of Esophageal Cancer Cells Under Flow. Scientific reports. 2016 Dec ; 6 38221. doi:10.1038/srep38221.

Bouyer C, Chen P, Güven S, Demirtaş TT, Nieland TJ, Padilla F, Demirci U. A Bio-Acoustic Levitational (BAL) Assembly Method for Engineering of Multilayered, 3D Brain-Like Constructs, Using Human Embryonic Stem Cell Derived Neuro-Progenitors. Advanced materials (Deerfield Beach, Fla.). 2016 Jan ; 28 (1): 161-7. doi:10.1002/adma.201503916.

Arslan-Yildiz A, El Assal R, Chen P, Guven S, Inci F, Demirci U. Towards artificial tissue models: past, present, and future of 3D bioprinting. Biofabrication. 2016 Mar ; 8 (1): 014103. doi:10.1088/1758-5090/8/1/014103.

Saxer F, Scherberich A, Todorov A, Studer P, Miot S, Schreiner S, Güven S, Tchang LA, Haug M, Heberer M, Schaefer DJ, Rikli D, Martin I, Jakob M. Implantation of Stromal Vascular Fraction Progenitors at Bone Fracture Sites: From a Rat Model to a First-in-Man Study. Stem cells (Dayton, Ohio). 2016 Dec ; 34 (12): 2956-2966. doi:10.1002/stem.2478.

Namkoong B, Güven S, Ramesan S, Liaudanskaya V, Abzhanov A, Demirci U. Recapitulating cranial osteogenesis with neural crest cells in 3-D microenvironments. Acta biomaterialia. 2016 Feb ; 31 301-311. doi:10.1016/j.actbio.2015.12.004.

Durmus NG, Tekin HC, Guven S, Sridhar K, Arslan Yildiz A, Calibasi G, Ghiran I, Davis RW, Steinmetz LM, Demirci U. Magnetic levitation of single cells. Proceedings of the National Academy of Sciences of the United States of America. 2015 Jul ; 112 (28): E3661-8. doi:10.1073/pnas.1509250112.

Guven S, Chen P, Inci F, Tasoglu S, Erkmen B, Demirci U. Multiscale assembly for tissue engineering and regenerative medicine. Trends in biotechnology. 2015 May ; 33 (5): 269-279. doi:10.1016/j.tibtech.2015.02.003.

Luo Z, Guven S, Gozen I, Chen P, Tasoglu S, Anchan RM, Bai B, Demirci U. Deformation of a single mouse oocyte in a constricted microfluidic channel. Microfluidics and nanofluidics. 2015 Oct ; 19 (4): 883-890. doi:10.1007/s10404-015-1614-0.

Tasoglu S, Yu CH, Liaudanskaya V, Guven S, Migliaresi C, Demirci U. Magnetic Levitational Assembly for Living Material Fabrication. Advanced healthcare materials. 2015 Jul ; 4 (10): 1469-76, 1422. doi:10.1002/adhm.201500092.

Chen P, Güven S, Usta OB, Yarmush ML, Demirci U. Biotunable acoustic node assembly of organoids. Advanced healthcare materials. 2015 Sep ; 4 (13): 1937-43. doi:10.1002/adhm.201500279.

Kaempfen A, Todorov A, Güven S, Largo RD, Jaquiéry C, Scherberich A, Martin I, Schaefer DJ. Engraftment of Prevascularized, Tissue Engineered Constructs in a Novel Rabbit Segmental Bone Defect Model. International journal of molecular sciences. 2015 Jun ; 16 (6): 12616-30. doi:10.3390/ijms160612616.

Guven S, Lindsey JS, Poudel I, Chinthala S, Nickerson MD, Gerami-Naini B, Gurkan UA, Anchan RM, Demirci U. Functional maintenance of differentiated embryoid bodies in microfluidic systems: a platform for personalized medicine. Stem cells translational medicine. 2015 Mar ; 4 (3): 261-8. doi:10.5966/sctm.2014-0119.

Tasoglu S, Diller E, Guven S, Sitti M, Demirci U. Untethered micro-robotic coding of three-dimensional material composition. Nature communications. 2014 Jan ; 5 3124. doi:10.1038/ncomms4124.

Tasoglu S, Yu CH, Gungordu HI, Guven S, Vural T, Demirci U. Guided and magnetic self-assembly of tunable magnetoceptive gels. Nature communications. 2014 Sep ; 5 4702. doi:10.1038/ncomms5702.

Chen P, Luo Z, Güven S, Tasoglu S, Ganesan AV, Weng A, Demirci U. Microscale assembly directed by liquid-based template. Advanced materials (Deerfield Beach, Fla.). 2014 Sep ; 26 (34): 5936-41. doi:10.1002/adma.201402079.

Klar AS, Güven S, Biedermann T, Luginbühl J, Böttcher-Haberzeth S, Meuli-Simmen C, Meuli M, Martin I, Scherberich A, Reichmann E. Tissue-engineered dermo-epidermal skin grafts prevascularized with adipose-derived cells. Biomaterials. 2014 Jun ; 35 (19): 5065-78. doi:10.1016/j.biomaterials.2014.02.049.

Tasoglu S, Kavaz D, Gurkan UA, Guven S, Chen P, Zheng R, Demirci U. Paramagnetic levitational assembly of hydrogels. Advanced materials (Deerfield Beach, Fla.). 2013 Feb ; 25 (8): 1137-43, 1081. doi:10.1002/adma.201200285.

Helmrich U, Di Maggio N, Güven S, Groppa E, Melly L, Largo RD, Heberer M, Martin I, Scherberich A, Banfi A. Osteogenic graft vascularization and bone resorption by VEGF-expressing human mesenchymal progenitors. Biomaterials. 2013 Jul ; 34 (21): 5025-35. doi:10.1016/j.biomaterials.2013.03.040.

Güven S, Karagianni M, Schwalbe M, Schreiner S, Farhadi J, Bula S, Bieback K, Martin I, Scherberich A. Validation of an automated procedure to isolate human adipose tissue-derived cells by using the Sepax® technology. Tissue engineering. Part C, Methods. 2012 Aug ; 18 (8): 575-82. doi:10.1089/ten.TEC.2011.0617.

Mehrkens A, Saxer F, Güven S, Hoffmann W, Müller AM, Jakob M, Weber FE, Martin I, Scherberich A. Intraoperative engineering of osteogenic grafts combining freshly harvested, human adipose-derived cells and physiological doses of bone morphogenetic protein-2. European cells & materials. 2012 Sep ; 24 308-19.

Güven S, Mehrkens A, Saxer F, Schaefer DJ, Martinetti R, Martin I, Scherberich A. Engineering of large osteogenic grafts with rapid engraftment capacity using mesenchymal and endothelial progenitors from human adipose tissue. Biomaterials. 2011 Sep ; 32 (25): 5801-9. doi:10.1016/j.biomaterials.2011.04.064.

Papadimitropoulos A, Scherberich A, Güven S, Theilgaard N, Crooijmans HJ, Santini F, Scheffler K, Zallone A, Martin I. A 3D in vitro bone organ model using human progenitor cells. European cells & materials. 2011 May ; 21 445-58; discussion 458.

Müller AM, Mehrkens A, Schäfer DJ, Jaquiery C, Güven S, Lehmicke M, Martinetti R, Farhadi I, Jakob M, Scherberich A, Martin I. Towards an intraoperative engineering of osteogenic and vasculogenic grafts from the stromal vascular fraction of human adipose tissue. European cells & materials. 2010 Mar ; 19 127-35.

Open Positions

PhD and MSc Positions in Therapeutic Bioengineering Group

Izmir Biomedicine and Genome Center Therapeutic Bioengineering Group is seeking for PhD and MSc student candidates to work on bioengineering projects.
Our group harmonizes fundamental sciences such as chemistry, biology, and physics with principles of engineering to generate innovative and effective therapeutic approaches, tissue mimicries and medical microdevices for medicine and pharmaceutical sciences. The focus of the group is to bioengineer novel native-like 3D cellular microenvironments, organ-on-a-chip platforms, and cell therapy approaches and to translate them into clinics.


Candidates should hold background or prior research experience in related fields such as biomedical engineering, tissue engineering, biomaterials, BioMEMS, microfluidics, stem cells and plant cell culture. Students graduated from departments of engineering (bioengineering, biomedical, mechanical, electrical, chemical) or basic sciences (molecular biology and genetics, biology, chemistry, physics) are welcomed to apply.

 
Applicants with strong academic record, English skills, self-motivation and dedication to a career in science/discovery are encouraged to contact Assoc. Prof. Sinan Güven (sinan.guven@ibg.edu.tr), http://ibg.edu.tr/research-programs/groups/guven-lab/.

Application dead line is July 5, 2019. Conditions can be found at http://ibg.deu.edu.tr.  

Awards

  • Sinan GUVEN - Young Scientist (BAGEP) Award by Science Academy, 2017
  • Sinan GUVEN - Aziz Sancar Encouragement Award by Health Institutes of Turkey (TUSEB), 2018
  • Canan Aslı YILDIRIM - Young Investigator (GEBİP) Awards by Turkish Academy of Sciences (TÜBA), 2018
  • Sinan GUVEN - Above-Threshold Award by TUBITAK, 2018
  • Sinan GUVEN - Young Investigator (GEBİP) Awards by Turkish Academy of Sciences (TÜBA), 2016

Contact

Guven Lab. on Therapeutic Bioengineering

Group Leader

Sinan GUVEN
sinan.guven@ibg.edu.tr
+90 232 299 41 00 (5131)
+9 02322994163